P-132: The Effect of Vitrification on Mouse Oocytes Survivale Rate and Apoptosis Using Cryotop
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Abstract:
Background: Vitrification of oocyte is one of the most important topics in the field of assisted reproductive techniques (ART). Considering the importance of oocyte vitrification in clinics, in the present study the effect of vitrification on mouse oocytes survivale rate and apoptosis by cryotop were investigated. Materials and Methods: 200 GV oocytes and 200 MII oocytes were obtained respectively from NMRI mouse ovaries and oviducts. GV and MII oocytes group, each again divided into two control and freezing groups. The oocytes in freezing group were vitrified after dehydration in eqilibration and vitrification media (Origio) using cryotop. Mouse oocytes survivale rate and apoptosis after staining with TUNEL technique were compared in control and freezing groups using Fisher’s exact test. Differences less than 0.05 were considered to be significant. Results: The survivale rates of GV and MII oocytes after vitrification and thawing was significantly different compared to control group (p<0.01 and p<0.01). The apoptosis rate of GV oocytes after vitrification and thawing did not show a significant difference compared to control group while the apoptosis rate of MII oocytes after vitrification and thawing was indicative of a significant difference compared to control group (p<0.001). Meanwhile, there was a significant difference in apoptosis rate between GV and MII oocytes after vitrification and thawing (p<0.03). Conclusion: The present study indicated that vitrification result in detrimental effect by reducing oocyte survivale rate and increasing of oocyte apoptosis.
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Journal title
volume 6 issue 2
pages -
publication date 2012-09-01
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